An E. coli-produced single-chain variable fragment (scFv) targeting hepatitis B virus surface protein potently inhibited virion secretion

Synthesis of a Soluble Flag-Tagged Single Chain Variable Fragment (scFv) Antibody Targeting Cucumber Mosaic Virus (CMV) Coat Protein

Cucumber mosaic virus (CMV) is a serious pathogen of many economically important commercial fruit and vegetable crops worldwide. It is a particular problem in warmer climates, where plants are not grown under cover thus necessitating undesirably high use of agrochemicals for the control of insect vectors. Efforts towards controlling of this virus would include the development of improved method...

Fragment (scFv) Specific for Intercellular Lactobacilli-Expressed Single-Chain Variable

RECOMBINANT SINGLE CHAIN VARIABLE FRAGMENT ANTIBODIES (scFv) AGAINST Pro144-Leu155 FRAGMENT OF HUMAN PROTEIN C.

The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from t...

Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit.

Previously we have described studies on in vitro pentamer assembly of Escherichia coli (E. coli) derived heat-labile enterotoxin B subunit (EtxB) using conventional monoclonal antibodies (Amin et al., JBC 1995: 270, 20143-50 and Chung et al., JBC 2006: 281, 39465-70). To extend these studies further we have used phage-display to select single-chain Fragment variable (scFv) antibodies against di...

Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries.

As bioinsecticides Bacillus thuringiensis Cry1C δ-endotoxins also have been used in genetically modified crops worldwide since last century. In this study, single chain variable fragments (scFvs), which could specifically recognize and detect Cry1C in food samples, were isolated from naive phage displayed human antibody libraries (Tomlinson I + J) by iterative affinity selection procedure inste...